Research Interests
Fatima Shafiee, a former BS student, worked on the in silico design and cloning of guide RNAs and homologous recombination (HR) templates for CRISPR-based editing of the endogenous URM1 locus. Her project focused on the PUB-UBL (Proximity Utilizing Biotinylation of Ubiquitin-like Modifiers) system to investigate the role of URM1 during DNA replication stress. She successfully performed molecular cloning to enable CRISPR-mediated tagging and functional analysis of this ubiquitin-like modifier.
Keywords
URM1, CRISPR-based genome editing, PUB-UBL